Outreach & Training
User training is at the core of our facility's mission, and we commit a significant amount of staff effort to training users in the application of standard and advanced macromolecular crystallographic techniques, with a particular focus on challenging time-resolved, Laue crystallography.
Another aspect of our mission is to keep present users, prospective users, and the broader scientific community well informed about capabilities and unique aspects of the facility, core research and development activities, and important scientific findings by our users and staff. To this end, BioCARS staff members actively disseminate our own and collaborative research by publishing in widely read, peer-reviewed journals, by contributing book chapters and review articles, and by participating in national and international scientific conferences.
- Time-resolved Macromolecular Crystallography (PDF) (Vukica Srajer)
- Laue Data Processing with Precognition (Zhong Ren)
- K. Moffat, Laue Crystallography: Time Resolved Studies, in International Tables for X-Ray Crystallography, Volume F: Crystallography of Biological Macromolecules, edited by M. G. Rossmann and E. Arnold. Ch. 8.2, p. 167-176 (2006)
- Schmidt, M, Structure-based kinetics by time-resolved X-ray crystallography, in Ultrashort Laser Pulses in Biology and Medicine (Markus Braun, Peter Gilch and Wolfgang Zinth, eds.), Springer Berlin Heidelberg, pp. 201-241 (2008)
- M. Schmidt, H. Ihee, R. Pahl and V. Srajer,
Protein-ligand interactions probed by time-resolved crystallography, in Protein-Ligand Interactions:
Methods and Applications (G. U. Nienhaus, ed.), Methods in Molecular Biology, vol. 305, Humana Press, Totawa, NJ, pp. 115-154 (2005)
- D. Bourgeois, M. Weik, Kinetic protein crystallography: a tool to watch proteins in action, Crystallography Reviews 15 (2) 87-118 (2009)
- Dominique Bourgeois, Friedrich Schotte, Maurizio Brunori, and Beatrice Vallone, Time-resolved methods in biophysics. 6. Time-resolved Laue crystallography
as a tool to investigate photo-activated protein dynamics, Photochem. Photobiol. Sci., 2007, 6, 1047–1056
- Dominique Bourgeois and Antoine Royant,
Advances in kinetic protein crystallography,
Current Opinion in Structural Biology 15 (5), 2005, 538-547
- M. Goeldner, R. Givens, eds., Dynamic Studies in Biology: Phototriggers, Photoswitches and Caged Biomolecules, Wiley-VCH, pp. 410–434 (2005).
- Crystallography 101
- Crystallography — CCP4 wiki
- IUCr Online Dictionary of Crystallography
- Teaching Crystallography
BioCARS staff members, together with users, contribute actively to dissemination of important scientific results based on the research conducted at the BioCARS facility. The major forms of dissemination are publications and participation at national and international conferences and workshops.
Most of training and educational activities in BioCARS are informal, daily, one-to-one interactions with our users and collaborators, but we periodically supplement this extensive informal training with more formal, workshop-type training. For example, a workshop we organized in 2006 focused on time-resolved and Laue techniques.
Because a major mission of the BioCARS facility is to develop time-resolved techniques and to train and expand the user community in this area of research, we commit significant time and effort to training new users in all aspects of the time-resolved technique (hardware, software, experimental procedures, data processing and analysis), and to keeping more experienced users up to date on the latest developments.
ACA Summer School in Crystallography
The American Crystallographic Association (ACA) Summer School in Crystallography offers another venue for providing training to a broader crystallographic community. The Summer School is conducted at the Illinois Institute of Technology (IIT) under the leadership of Andy Howard, IIT. It provides two weeks of concentrated, lecture-based training, as well as practical, hands-on training on crystallization, data collection at synchrotron facilities such as BioCARS, and use of crystallographic software for data processing and structure solving. We have actively participated in the School for several years, contributing beam time (several days each summer) and staff time, both for hands-on training in standard macromolecular crystallography methods and for lectures on Laue and time-resolved crystallography).
We give tours of the BioCARS facility frequently and to diverse groups of visitors ranging from members of the general public and high school students to University of Chicago alumni. In 2007, for example, more than 160 visitors toured our facility, most of them students.